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KMID : 0360319940260020296
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Journal of Korean Cancer Research Association
1994 Volume.26 No. 2 p.296 ~ p.303
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Mutations of the p53 Tumor Suppressor Gene in Human Epithelial Ovarian Cancer
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김진우
권동진/나덕진/이준모/김대훈/남궁성은/김승조
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Abstract
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The p53 gene on chromosome 17p is considered to be a tumor suppressor gene, and frequent mutations in highly conserved regions of the p53 gene have been found in a wide variety of human cancers. Mutations in this portion of the gene are known to
lead to
the loss of the oncosuppressive potential of p53.
To detect in a more sensitive manner p53 gene mutations in 28 human epithelial ovarian cancers we utilized the polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) technique.
Using PCR primers for the regions of the p53 gene, including exons 4-9, p53 mutations were detected in 35.7%(10 of 28) of the ovarian cancers. Of the 10 mutations observed, 1 was found in exon 4, 7 were found in the region encompassing exons 5
and
6, 1
was found in exon 7 and 1 was found int he region encompassing exons 8 and 9. Mutations were clustered in exons 5 and 6 in highly conserved regions of the p53 gene.
There results suggest that the mutations of the p53 gene play an important role in the development of human epthelial ovarian cancers.
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KEYWORD
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